WGA1

Sigma

 

GenomePlex® Whole Genome Amplification (WGA) Kit

Description

Frequently Asked QuestionsLive Chat and Frequently Asked Questions are available for this Product.
Features and Benefits• Flexibility to study DNA from any source
• No detectable locus or allele bias
• Low background amplification of DNA from single chromosomes and cells
• Compatible with a variety of microarray, capillary, and homogenous platforms for sequencing, genotyping, CGH, FISH, ChIP
• Increased sensitivity and accuracy for population studies, mutation discovery, and pharmacogenomics.
General descriptionGenomePlex Whole Genome Amplification (WGA) Kit utilizes a proprietary technology based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers.
 WGA is suitable for use with genomic DNA, whole blood, buffy coats, buccal swabs, and cultured cells. Amplification requires only a small amount of starting material (1 μL blood, single cheek swab, or 10 ng of gDNA), which after PCR produces a yield of 5 - 10 μg per reaction. The amplified DNA can be purified and stored or used for downstream analyses including ABI's TaqMan® assays, microsatellite analysis, or sequencing. GenomePlex gives a complete representation of the entire genome with no detectable allele bias.
Legal InformationThis product is for research use only. Use of this product for human or animal therapeutic or diagnostic purposes requires licensing from Rubicon Genomics, Inc. Unauthorized use violates proprietary rights of Rubicon Genomics, Inc.
 TaqMan is a registered trademark of Roche Molecular Systems, Inc.
 GenomePlex is a registered trademark of Rubicon Genomics, Inc.

Properties

shipped inwet ice
storage temp.−20°C

Safety

Hazard CodesXi,B
Risk Statements36/37/38
Safety Statements26-36

Related Products

Related productNA1020, GenElute™ PCR Clean-Up Kit
 Z719218, Whole Genome Amplification: Methods Express Series

References

referenceBarker, D. L., et al., Two methods for whole-genome amplification enable accurate genotyping across a 2320-SNP linkage panel Genome Res. 14, 901-907, (2004) Abstract
 Gribble, S., et al., Chromosome paints from single copies of chromosomes Chromosome Res. 12, 143-151, (2004) Abstract
 Thorstenson, Y. R. et al., An Automated Hydrodynamic Process for Controlled, Unbiased DNA Shearing Genome Res. 8, 848-855, (1998) Abstract
 Park, J. W., et al., Comparing Whole-Genome Amplification Methods and Sources for Biological Samples for Single-Nucleotide Polymorphism Genotyping Clin. Chem. 51(8), 1520-1523, (2004)